Extraction:Article Title: Effects of gestational perfluorohexanesulfonic acid exposure at human realistic dose on social communication deficit in mouse offspring.
Article Snippet: .. For extraction of metabolites, 800 μL of extraction solvents (acetonitrile/ methanol, v/v = 1/1; 1000291000, Merck, USA; CAS No. 75-05-8; ≥99.9%) containing L-Phenyl-d5-alanine2,3,3-d3 internal standard (5 μg/mL; HY-N0215S12, Toronto Research Chemicals, Canada) was added to 200 μL homogenate, the mixture was vortexed for 5 min. After incubation at −20 ◦ C for 2 h and centrifugation for 10 min (14,000 rpm, 4 ◦ C), the supernatant was collected and then dried under gentle nitrogen flow, which was later reconstituted in 150 μL solvent (acetonitrile/water, v/v = 1/1) for LC-MS/MS analysis. .. Chromatographic separation was performed on an ACQUITY Premier HSS T3 column (1.8 μm, 2.1 × 150 mm; Waters ACQUITY UPLC, USA).
Article Title: Valorization of Plastic Waste: A Lab-Scale Approach with the Aid of Solar Hydrothermal Liquefaction Technology
Article Snippet: Hydrothermal liquefaction (HTL) technology could potentially be utilized for the conversion of non-recyclable plastics emanating from process streams into added value products, exploiting the feedstock moisture.. Although solar heat and HTL coupling is a promising prospect for reducing the high energy requirements of the process, it is not sufficiently developed.. The present study aims to investigate the product properties and biocrude yield resulting from HTL of plastic waste mix and polypropylene at the lab scale by conventional heating and solar energy-aided.
Article Title: Effects of gestational perfluorohexanesulfonic acid exposure at human realistic dose on social communication deficit in mouse offspring
Article Snippet: .. For extraction of metabolites, 800 μL of extraction solvents (acetonitrile/methanol, v/v = 1/1; 1000291000, Merck, USA; CAS No. 75-05-8; ≥99.9%) containing L-Phenyl-d5-alanine-2,3,3-d3 internal standard (5 μg/mL; HY-N0215S12, Toronto Research Chemicals, Canada) was added to 200 μL homogenate, the mixture was vortexed for 5 min. After incubation at −20 °C for 2 h and centrifugation for 10 min (14,000 rpm, 4 °C), the supernatant was collected and then dried under gentle nitrogen flow, which was later reconstituted in 150 μL solvent (acetonitrile/water, v/v = 1/1) for LC-MS/MS analysis. .. Chromatographic separation was performed on an ACQUITY Premier HSS T3 column (1.8 μm, 2.1 × 150 mm; Waters ACQUITY UPLC, USA).
Incubation:Article Title: Effects of gestational perfluorohexanesulfonic acid exposure at human realistic dose on social communication deficit in mouse offspring.
Article Snippet: .. For extraction of metabolites, 800 μL of extraction solvents (acetonitrile/ methanol, v/v = 1/1; 1000291000, Merck, USA; CAS No. 75-05-8; ≥99.9%) containing L-Phenyl-d5-alanine2,3,3-d3 internal standard (5 μg/mL; HY-N0215S12, Toronto Research Chemicals, Canada) was added to 200 μL homogenate, the mixture was vortexed for 5 min. After incubation at −20 ◦ C for 2 h and centrifugation for 10 min (14,000 rpm, 4 ◦ C), the supernatant was collected and then dried under gentle nitrogen flow, which was later reconstituted in 150 μL solvent (acetonitrile/water, v/v = 1/1) for LC-MS/MS analysis. .. Chromatographic separation was performed on an ACQUITY Premier HSS T3 column (1.8 μm, 2.1 × 150 mm; Waters ACQUITY UPLC, USA).
Article Title: Effects of gestational perfluorohexanesulfonic acid exposure at human realistic dose on social communication deficit in mouse offspring
Article Snippet: .. For extraction of metabolites, 800 μL of extraction solvents (acetonitrile/methanol, v/v = 1/1; 1000291000, Merck, USA; CAS No. 75-05-8; ≥99.9%) containing L-Phenyl-d5-alanine-2,3,3-d3 internal standard (5 μg/mL; HY-N0215S12, Toronto Research Chemicals, Canada) was added to 200 μL homogenate, the mixture was vortexed for 5 min. After incubation at −20 °C for 2 h and centrifugation for 10 min (14,000 rpm, 4 °C), the supernatant was collected and then dried under gentle nitrogen flow, which was later reconstituted in 150 μL solvent (acetonitrile/water, v/v = 1/1) for LC-MS/MS analysis. .. Chromatographic separation was performed on an ACQUITY Premier HSS T3 column (1.8 μm, 2.1 × 150 mm; Waters ACQUITY UPLC, USA).
Centrifugation:Article Title: Effects of gestational perfluorohexanesulfonic acid exposure at human realistic dose on social communication deficit in mouse offspring.
Article Snippet: .. For extraction of metabolites, 800 μL of extraction solvents (acetonitrile/ methanol, v/v = 1/1; 1000291000, Merck, USA; CAS No. 75-05-8; ≥99.9%) containing L-Phenyl-d5-alanine2,3,3-d3 internal standard (5 μg/mL; HY-N0215S12, Toronto Research Chemicals, Canada) was added to 200 μL homogenate, the mixture was vortexed for 5 min. After incubation at −20 ◦ C for 2 h and centrifugation for 10 min (14,000 rpm, 4 ◦ C), the supernatant was collected and then dried under gentle nitrogen flow, which was later reconstituted in 150 μL solvent (acetonitrile/water, v/v = 1/1) for LC-MS/MS analysis. .. Chromatographic separation was performed on an ACQUITY Premier HSS T3 column (1.8 μm, 2.1 × 150 mm; Waters ACQUITY UPLC, USA).
Article Title: Effects of gestational perfluorohexanesulfonic acid exposure at human realistic dose on social communication deficit in mouse offspring
Article Snippet: .. For extraction of metabolites, 800 μL of extraction solvents (acetonitrile/methanol, v/v = 1/1; 1000291000, Merck, USA; CAS No. 75-05-8; ≥99.9%) containing L-Phenyl-d5-alanine-2,3,3-d3 internal standard (5 μg/mL; HY-N0215S12, Toronto Research Chemicals, Canada) was added to 200 μL homogenate, the mixture was vortexed for 5 min. After incubation at −20 °C for 2 h and centrifugation for 10 min (14,000 rpm, 4 °C), the supernatant was collected and then dried under gentle nitrogen flow, which was later reconstituted in 150 μL solvent (acetonitrile/water, v/v = 1/1) for LC-MS/MS analysis. .. Chromatographic separation was performed on an ACQUITY Premier HSS T3 column (1.8 μm, 2.1 × 150 mm; Waters ACQUITY UPLC, USA).
Gentle:Article Title: Effects of gestational perfluorohexanesulfonic acid exposure at human realistic dose on social communication deficit in mouse offspring.
Article Snippet: .. For extraction of metabolites, 800 μL of extraction solvents (acetonitrile/ methanol, v/v = 1/1; 1000291000, Merck, USA; CAS No. 75-05-8; ≥99.9%) containing L-Phenyl-d5-alanine2,3,3-d3 internal standard (5 μg/mL; HY-N0215S12, Toronto Research Chemicals, Canada) was added to 200 μL homogenate, the mixture was vortexed for 5 min. After incubation at −20 ◦ C for 2 h and centrifugation for 10 min (14,000 rpm, 4 ◦ C), the supernatant was collected and then dried under gentle nitrogen flow, which was later reconstituted in 150 μL solvent (acetonitrile/water, v/v = 1/1) for LC-MS/MS analysis. .. Chromatographic separation was performed on an ACQUITY Premier HSS T3 column (1.8 μm, 2.1 × 150 mm; Waters ACQUITY UPLC, USA).
Article Title: Effects of gestational perfluorohexanesulfonic acid exposure at human realistic dose on social communication deficit in mouse offspring
Article Snippet: .. For extraction of metabolites, 800 μL of extraction solvents (acetonitrile/methanol, v/v = 1/1; 1000291000, Merck, USA; CAS No. 75-05-8; ≥99.9%) containing L-Phenyl-d5-alanine-2,3,3-d3 internal standard (5 μg/mL; HY-N0215S12, Toronto Research Chemicals, Canada) was added to 200 μL homogenate, the mixture was vortexed for 5 min. After incubation at −20 °C for 2 h and centrifugation for 10 min (14,000 rpm, 4 °C), the supernatant was collected and then dried under gentle nitrogen flow, which was later reconstituted in 150 μL solvent (acetonitrile/water, v/v = 1/1) for LC-MS/MS analysis. .. Chromatographic separation was performed on an ACQUITY Premier HSS T3 column (1.8 μm, 2.1 × 150 mm; Waters ACQUITY UPLC, USA).
Solvent:Article Title: Effects of gestational perfluorohexanesulfonic acid exposure at human realistic dose on social communication deficit in mouse offspring.
Article Snippet: .. For extraction of metabolites, 800 μL of extraction solvents (acetonitrile/ methanol, v/v = 1/1; 1000291000, Merck, USA; CAS No. 75-05-8; ≥99.9%) containing L-Phenyl-d5-alanine2,3,3-d3 internal standard (5 μg/mL; HY-N0215S12, Toronto Research Chemicals, Canada) was added to 200 μL homogenate, the mixture was vortexed for 5 min. After incubation at −20 ◦ C for 2 h and centrifugation for 10 min (14,000 rpm, 4 ◦ C), the supernatant was collected and then dried under gentle nitrogen flow, which was later reconstituted in 150 μL solvent (acetonitrile/water, v/v = 1/1) for LC-MS/MS analysis. .. Chromatographic separation was performed on an ACQUITY Premier HSS T3 column (1.8 μm, 2.1 × 150 mm; Waters ACQUITY UPLC, USA).
Article Title: Effects of gestational perfluorohexanesulfonic acid exposure at human realistic dose on social communication deficit in mouse offspring
Article Snippet: .. For extraction of metabolites, 800 μL of extraction solvents (acetonitrile/methanol, v/v = 1/1; 1000291000, Merck, USA; CAS No. 75-05-8; ≥99.9%) containing L-Phenyl-d5-alanine-2,3,3-d3 internal standard (5 μg/mL; HY-N0215S12, Toronto Research Chemicals, Canada) was added to 200 μL homogenate, the mixture was vortexed for 5 min. After incubation at −20 °C for 2 h and centrifugation for 10 min (14,000 rpm, 4 °C), the supernatant was collected and then dried under gentle nitrogen flow, which was later reconstituted in 150 μL solvent (acetonitrile/water, v/v = 1/1) for LC-MS/MS analysis. .. Chromatographic separation was performed on an ACQUITY Premier HSS T3 column (1.8 μm, 2.1 × 150 mm; Waters ACQUITY UPLC, USA).
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